Old meets new: most probable number validation of metagenomic and metatranscriptomic datasets in soil
نویسندگان
چکیده
منابع مشابه
The Most Probable Number Method and Its Uses in Enumeration, Qualification, and Validation
"Microbiology Topics" discusses various topics in microbiology of practical use in validation and com pliance. We intend this column to be a useful resource for daily work applications. Reader comments, questions, and suggestions are needed to help us fulfill our objective for this col umn. Please send your comments and suggestions to column coordinator Scott Sutton at scott.sutton@ microbiol...
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The fungus Polymyxa betae is an obligate parasite of the roots of many plants of the family Chenopodiaceae. In the sugar beet, it acts as a vector of beet necrotic yellow vein virus, the agent of a serious disease known as rhizomania. With indirect methods of analysis, such as bioassay, one can establish only the presence or absence, but not the quantity, of P. betae in soil. A new method based...
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The design of most-probable-number (MPN) experiments is restricted by the availability and completeness of tables for certain dilution ratio and replicate number combinations. The results presented in this paper are from a computer program, the Most Probable Number Enumeration System (MPNES), which generates solutions and confidence limits (P = 0.05) for population estimates of MPN data. Tables...
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This paper reports on the first successful molecular detection and quantification of soil protozoa. Quantification of heterotrophic flagellates and naked amoebae in soil has traditionally relied on dilution culturing techniques, followed by most-probable-number (MPN) calculations. Such methods are biased by differences in the culturability of soil protozoa and are unable to quantify specific ta...
متن کاملa fundamental study of "histiriographic metafiction", and "literary genres", as introduced in "new historical philosophy", and tracing them in the works of julian barnes.
abstract a fundamental study of “historio-graphic metafiction” and “literary genres”, as introduced in “new historical philosophy”, and tracing them in the works of julian barnes having studied the two novels, the porcupine and arthur & george, by julian barnes, the researcher has applied linda hutcheon’s historio-graphic metafictional theories to them. the thesis is divided into five cha...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2017
ISSN: 0266-8254
DOI: 10.1111/lam.12821